Linearity and LoQ were determined using Analyse-it software3. Linearity of the assay was assessed by linear and polynomial regression of the data and difference plot with Total Error criteria. A Weighted Deming fit was used to compare the fit between the hscTnT assay versus the cTnI assay. Regardless, a hscTnT 6.6 ng/L cut-off point differentiating healthy from those with primary myocardial is likely more appropriately applied to relatively sedentary horses rather than racing populations. About RocheHeadquartered in Basel, Switzerland, Roche is a leader in research-focused healthcare with combined strengths in pharmaceuticals and diagnostics. Triplicates of the resultant 11 samples (mean hscTnT levels ranging from 8.0 to 3526.7 ng/L) were analyzed to detect linearity over the expected measurable range.
Linear dose response N = 10 dilutions were prepared from purified equine cardiac troponin stock by serial 1:2 dilution (from 1:100 to 1:0.2) using normal equine plasma (hscTnT 3.28 ng/L) as diluent. For both populations, a physical examination with heart auscultation was performed, and Telemetric ECG was run stall-side and observed for two minutes on all horses. Eur Heart J. 2012;33(20):2551–67.View ArticlePubMedGoogle ScholarApple FS, Collinson PO. Biomarkers ITFoCAoC: analytical characteristics of high-sensitivity cardiac troponin assays. Founded in 1896, Roche has been making important contributions to global health for more than a century.
Degree of hemolysis in all samples was assessed using the serum indices instrument application on the Roche Cobas-e601 platform1 and no specimen included in this study reached the hemolysis threshold to be excluded. Catalog Number: 04491815190 INTENDED USE Immunoassay for the in vitro quantitative determination of troponin T in human serum and plasma. Criteria for significant change: average difference of less than 2X the between-run standard deviation of the control level closest to the mean of samples measured. The gelding finished the 0.5 mile race in distress and presented immediately for assessment.